ISCLB 2024


The wheat pathogen, Zymoseptoria tritici, expresses effectors that bind lipid transfer proteins (LTPs; PR-14)

Eli Thynne

on  Th, 16:50 ! Livein  CHN C14 (conference room)for  20min

Before inducing necrotic disease symptoms, the wheat pathogen, Zymoseptoria tritici, undergoes an asymptomatic growth phase of almost two weeks. During this time, the pathogen grows throughout the host’s apoplast, evading immune responses. However, the wheat immune system is not dormant, and numerous immune-related genes are highly expressed while Z. tritici infects. Among these are lipid transfer proteins (LTPs; PR-14).LTPs are highly abundant apoplastic proteins during Z. tritici’s infection, and can function as antifungals. They have also been reported as immune-signaling molecules, associating with hormones, and another PR protein, PR-1, to enhance resistance. Despite this, Z. tritici can tolerate the presence of LTPs during infection. We have identified a class of effectors expressed by Z. tritici that can interact directly with LTPs. We are exploring if these effectors can inhibit LTPs’ antifungal activity and their interaction with PR-1. Transient expression of these effectors in the non-host, Nicotiana benthamiana, can lead to cell-death. Interestingly, N. benthamiana has a receptor-like protein (RLP), that interacts with LTPs coplexed with jasmonic acid. Silencing of this RLP (or the RLP co-receptor SOBIR1), inhibits LTP-binding effector-induced cell-death, indicating that it is responsible for this non-host effector recognition. This RLP could potentially represent a source of disease resistance to fungal pathogens that express this class of LTP-binding effectors.

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