ISCLB 2024


Exploring the role of the Parastagonospora nodorum necrotrophic effector, SnTox267, during in planta colonization

Ashley Nelson

on  Sa, 14:15 ! Livein  HG D1.1 (conference room)for  20min

Parastagonospora nodorum is a necrotrophic fungal pathogen that causes septoria nodorum blotch (SNB) on wheat. There have been five necrotrophic effectors identified, cloned, and found to contribute to P. nodorum virulence. The necrotrophic effector SnTox267 was originally thought to be three individual effectors (i.e., SnTox2, SnTox6 and SnTox7) that targeted wheat susceptibility genes Snn2, Snn6 and Snn7, respectively. However, recently it was discovered that SnTox267 is a single proteinaceous necrotrophic effector, targeting at least two separate pathways in wheat. Here we characterize in-planta colonization by comparing a wild type P. nodorum isolate, Sn4 with its SnTox267 disruption strain (Sn4Δtox267) on wheat lines BG284 (SnTox267 insensitive), BG223 (SnTox267 sensitive) and Grandin (SnTox267 sensitive). Samples were stained using fluorescent dye, analyzed using confocal microscopy, and quantified using machine learning assisted volume analysis. In addition to this, reactive oxygen species production was measured using 3-3’-Diaminobenzene (DAB) staining, which is oxidized by hydrogen peroxide (H2O2), and nitroblue tetrazolium (NBT) staining, which is oxidized by superoxide anions (O2-). Using these, we can begin to characterize the temporal and spatial role of SnTox267 in planta.

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